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min6 cell apoptosis rate  (Proteintech)


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    Proteintech min6 cell apoptosis rate
    Exosome isolation, identification and tracing. ( A ) Representative images of the isolated exosomes observed by TEM. Bar = 100μm. ( B ) WB showed the biomarkers level of CD 9, ALIX and TSG 101 in the isolated exosomes. ( C ) The size and amount of extracellular particles measured by NTA. ( D ) PKH67-labeled exosomes were absorbed by <t>Min6</t> observed by a confocal microscope (Nikon, Tokyo, Japan). Bar = 50μm. The NCEXO ( E ) and PAEXO ( F ) tracing in vivo was evaluated by the in vivo imaging system (Bruker MI SE 7.2 software, Bruker, German). The fluorescence images were taken at day 1, 2, 3, 5.
    Min6 Cell Apoptosis Rate, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 2375 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/min6+cell+apoptosis+rate/CEL+Antibody/pmc11144429-77-5-19
    Average 96 stars, based on 2375 article reviews
    min6 cell apoptosis rate - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Small Intestinal Endocrine Cell Derived Exosomal ACE2 Protects Islet β-Cell Function by Inhibiting the Activation of NLRP3 Inflammasome and Reducing β-Cell Pyroptosis"

    Article Title: Small Intestinal Endocrine Cell Derived Exosomal ACE2 Protects Islet β-Cell Function by Inhibiting the Activation of NLRP3 Inflammasome and Reducing β-Cell Pyroptosis

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S450337

    Exosome isolation, identification and tracing. ( A ) Representative images of the isolated exosomes observed by TEM. Bar = 100μm. ( B ) WB showed the biomarkers level of CD 9, ALIX and TSG 101 in the isolated exosomes. ( C ) The size and amount of extracellular particles measured by NTA. ( D ) PKH67-labeled exosomes were absorbed by Min6 observed by a confocal microscope (Nikon, Tokyo, Japan). Bar = 50μm. The NCEXO ( E ) and PAEXO ( F ) tracing in vivo was evaluated by the in vivo imaging system (Bruker MI SE 7.2 software, Bruker, German). The fluorescence images were taken at day 1, 2, 3, 5.
    Figure Legend Snippet: Exosome isolation, identification and tracing. ( A ) Representative images of the isolated exosomes observed by TEM. Bar = 100μm. ( B ) WB showed the biomarkers level of CD 9, ALIX and TSG 101 in the isolated exosomes. ( C ) The size and amount of extracellular particles measured by NTA. ( D ) PKH67-labeled exosomes were absorbed by Min6 observed by a confocal microscope (Nikon, Tokyo, Japan). Bar = 50μm. The NCEXO ( E ) and PAEXO ( F ) tracing in vivo was evaluated by the in vivo imaging system (Bruker MI SE 7.2 software, Bruker, German). The fluorescence images were taken at day 1, 2, 3, 5.

    Techniques Used: Isolation, Labeling, Microscopy, In Vivo, In Vivo Imaging, Software, Fluorescence

    ACE2-riched NCEXO regulates proliferation, apoptosis and inflammation of islets β-cells in vitro. ( A and B ) WB analysis revealed that NCEXO exhibited higher levels of ACE2 compared to PAEXO. ( C ) Viability assessment of Min6 cells under varying concentrations of exosomes using CCK-8. ( D ) Flow cytometry analysis of Min6 cells apoptosis rate. Unit (%) ( E ) Min6 cell proliferation was determined with an EDU Kit. Bar = 100μm ( F and H ) Protein expression of Bax, Bcl-2, Caspase-3, Cleaved Caspase-3, NFκB and P- NFκB in MIN6 cells and densitometric quantification. ( G ) Real-time PCR analysis of Bax, Bcl-2, Caspase-3, NFκB, PDX1 of Min6 cell. ( I ) Min6 cell insulin secretion ability was measured by GSIS. *P < 0.05, **P < 0.01 and ***P < 0.001; # P < 0.05 NCEXO group vs PA group.
    Figure Legend Snippet: ACE2-riched NCEXO regulates proliferation, apoptosis and inflammation of islets β-cells in vitro. ( A and B ) WB analysis revealed that NCEXO exhibited higher levels of ACE2 compared to PAEXO. ( C ) Viability assessment of Min6 cells under varying concentrations of exosomes using CCK-8. ( D ) Flow cytometry analysis of Min6 cells apoptosis rate. Unit (%) ( E ) Min6 cell proliferation was determined with an EDU Kit. Bar = 100μm ( F and H ) Protein expression of Bax, Bcl-2, Caspase-3, Cleaved Caspase-3, NFκB and P- NFκB in MIN6 cells and densitometric quantification. ( G ) Real-time PCR analysis of Bax, Bcl-2, Caspase-3, NFκB, PDX1 of Min6 cell. ( I ) Min6 cell insulin secretion ability was measured by GSIS. *P < 0.05, **P < 0.01 and ***P < 0.001; # P < 0.05 NCEXO group vs PA group.

    Techniques Used: In Vitro, CCK-8 Assay, Flow Cytometry, Expressing, Real-time Polymerase Chain Reaction

    The protective effect of NCEXO primarily stems from its ACE2. ( A ) Protein expression of NLRP3, ASC, Caspase-1, Cleaved Caspase-1, Pro-ILβ, IL1β, IL18, GSDMD and N- GSDMD in mouse pancreatic islets and densitometric quantification. ( B ) Quantitative Real-time PCR was used to measure the level of NLRP3, ASC, Caspase-1, IL1β, IL18, GSDMD of mouse pancreatic islets. ( C ) Quantitative Real-time PCR was used to measure the level of NLRP3, ASC, Caspase-1, IL1β, IL18, GSDMD of Min6 cell. ( D ) The densitometric quantification of Protein expression of NLRP3, ASC, Caspase-1, Cleaved Caspase-1, Pro-ILβ, IL1β, IL18, GSDMD and N- GSDMD in mouse pancreatic islets. ( E ) Quantitative Real-time PCR was used to measure the level of FOXO1, NKX6.1, PDX1, ACE2, NFκB, MAFA of Min6 cell. ( F ) Effect of A779/A1-7 on Min6’cells proliferation measured by EDU. Bar = 100μm ( G-I ) Protein expression of FOXO1, NKX6.1, PDX1, IκB, P-IκB, NFκB, P- NFκB, NLRP3, ASC, Caspase-1, Cleaved Caspase-1, Pro-ILβ, IL1β, IL18, GSDMD and N- GSDMD in Min6 cell. *P < 0.05, **P < 0.01 and ***P < 0.001.
    Figure Legend Snippet: The protective effect of NCEXO primarily stems from its ACE2. ( A ) Protein expression of NLRP3, ASC, Caspase-1, Cleaved Caspase-1, Pro-ILβ, IL1β, IL18, GSDMD and N- GSDMD in mouse pancreatic islets and densitometric quantification. ( B ) Quantitative Real-time PCR was used to measure the level of NLRP3, ASC, Caspase-1, IL1β, IL18, GSDMD of mouse pancreatic islets. ( C ) Quantitative Real-time PCR was used to measure the level of NLRP3, ASC, Caspase-1, IL1β, IL18, GSDMD of Min6 cell. ( D ) The densitometric quantification of Protein expression of NLRP3, ASC, Caspase-1, Cleaved Caspase-1, Pro-ILβ, IL1β, IL18, GSDMD and N- GSDMD in mouse pancreatic islets. ( E ) Quantitative Real-time PCR was used to measure the level of FOXO1, NKX6.1, PDX1, ACE2, NFκB, MAFA of Min6 cell. ( F ) Effect of A779/A1-7 on Min6’cells proliferation measured by EDU. Bar = 100μm ( G-I ) Protein expression of FOXO1, NKX6.1, PDX1, IκB, P-IκB, NFκB, P- NFκB, NLRP3, ASC, Caspase-1, Cleaved Caspase-1, Pro-ILβ, IL1β, IL18, GSDMD and N- GSDMD in Min6 cell. *P < 0.05, **P < 0.01 and ***P < 0.001.

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction

    Related Articles

    Flow Cytometry:

    Article Title: Small Intestinal Endocrine Cell Derived Exosomal ACE2 Protects Islet β-Cell Function by Inhibiting the Activation of NLRP3 Inflammasome and Reducing β-Cell Pyroptosis
    Article Snippet: Cell viability was assessed by a CCK-8 (Dojindo Laboratories, Kumamoto, Japan) assay according to the manufacturer’s protocol after the cell treatment. .. After specific cell treatment, the Min6 cell apoptosis rate was performed using an Annexin V and PI apoptosis kit (Proteintech, PF00005) according to the manufacturer’s protocol and then measured by flow cytometry (Becton, Franklin Lake, NJ, USA). .. The results were analyzed using FlowJov.10.6.2 software (LLC, Ashland, USA)



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    min6 cell apoptosis rate  (Proteintech)
    96
    Proteintech min6 cell apoptosis rate
    Exosome isolation, identification and tracing. ( A ) Representative images of the isolated exosomes observed by TEM. Bar = 100μm. ( B ) WB showed the biomarkers level of CD 9, ALIX and TSG 101 in the isolated exosomes. ( C ) The size and amount of extracellular particles measured by NTA. ( D ) PKH67-labeled exosomes were absorbed by <t>Min6</t> observed by a confocal microscope (Nikon, Tokyo, Japan). Bar = 50μm. The NCEXO ( E ) and PAEXO ( F ) tracing in vivo was evaluated by the in vivo imaging system (Bruker MI SE 7.2 software, Bruker, German). The fluorescence images were taken at day 1, 2, 3, 5.
    Min6 Cell Apoptosis Rate, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/min6+cell+apoptosis+rate/CEL+Antibody/pmc11144429-77-5-19
    Average 96 stars, based on 1 article reviews
    min6 cell apoptosis rate - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Exosome isolation, identification and tracing. ( A ) Representative images of the isolated exosomes observed by TEM. Bar = 100μm. ( B ) WB showed the biomarkers level of CD 9, ALIX and TSG 101 in the isolated exosomes. ( C ) The size and amount of extracellular particles measured by NTA. ( D ) PKH67-labeled exosomes were absorbed by Min6 observed by a confocal microscope (Nikon, Tokyo, Japan). Bar = 50μm. The NCEXO ( E ) and PAEXO ( F ) tracing in vivo was evaluated by the in vivo imaging system (Bruker MI SE 7.2 software, Bruker, German). The fluorescence images were taken at day 1, 2, 3, 5.

    Journal: International Journal of Nanomedicine

    Article Title: Small Intestinal Endocrine Cell Derived Exosomal ACE2 Protects Islet β-Cell Function by Inhibiting the Activation of NLRP3 Inflammasome and Reducing β-Cell Pyroptosis

    doi: 10.2147/IJN.S450337

    Figure Lengend Snippet: Exosome isolation, identification and tracing. ( A ) Representative images of the isolated exosomes observed by TEM. Bar = 100μm. ( B ) WB showed the biomarkers level of CD 9, ALIX and TSG 101 in the isolated exosomes. ( C ) The size and amount of extracellular particles measured by NTA. ( D ) PKH67-labeled exosomes were absorbed by Min6 observed by a confocal microscope (Nikon, Tokyo, Japan). Bar = 50μm. The NCEXO ( E ) and PAEXO ( F ) tracing in vivo was evaluated by the in vivo imaging system (Bruker MI SE 7.2 software, Bruker, German). The fluorescence images were taken at day 1, 2, 3, 5.

    Article Snippet: After specific cell treatment, the Min6 cell apoptosis rate was performed using an Annexin V and PI apoptosis kit (Proteintech, PF00005) according to the manufacturer’s protocol and then measured by flow cytometry (Becton, Franklin Lake, NJ, USA).

    Techniques: Isolation, Labeling, Microscopy, In Vivo, In Vivo Imaging, Software, Fluorescence

    ACE2-riched NCEXO regulates proliferation, apoptosis and inflammation of islets β-cells in vitro. ( A and B ) WB analysis revealed that NCEXO exhibited higher levels of ACE2 compared to PAEXO. ( C ) Viability assessment of Min6 cells under varying concentrations of exosomes using CCK-8. ( D ) Flow cytometry analysis of Min6 cells apoptosis rate. Unit (%) ( E ) Min6 cell proliferation was determined with an EDU Kit. Bar = 100μm ( F and H ) Protein expression of Bax, Bcl-2, Caspase-3, Cleaved Caspase-3, NFκB and P- NFκB in MIN6 cells and densitometric quantification. ( G ) Real-time PCR analysis of Bax, Bcl-2, Caspase-3, NFκB, PDX1 of Min6 cell. ( I ) Min6 cell insulin secretion ability was measured by GSIS. *P < 0.05, **P < 0.01 and ***P < 0.001; # P < 0.05 NCEXO group vs PA group.

    Journal: International Journal of Nanomedicine

    Article Title: Small Intestinal Endocrine Cell Derived Exosomal ACE2 Protects Islet β-Cell Function by Inhibiting the Activation of NLRP3 Inflammasome and Reducing β-Cell Pyroptosis

    doi: 10.2147/IJN.S450337

    Figure Lengend Snippet: ACE2-riched NCEXO regulates proliferation, apoptosis and inflammation of islets β-cells in vitro. ( A and B ) WB analysis revealed that NCEXO exhibited higher levels of ACE2 compared to PAEXO. ( C ) Viability assessment of Min6 cells under varying concentrations of exosomes using CCK-8. ( D ) Flow cytometry analysis of Min6 cells apoptosis rate. Unit (%) ( E ) Min6 cell proliferation was determined with an EDU Kit. Bar = 100μm ( F and H ) Protein expression of Bax, Bcl-2, Caspase-3, Cleaved Caspase-3, NFκB and P- NFκB in MIN6 cells and densitometric quantification. ( G ) Real-time PCR analysis of Bax, Bcl-2, Caspase-3, NFκB, PDX1 of Min6 cell. ( I ) Min6 cell insulin secretion ability was measured by GSIS. *P < 0.05, **P < 0.01 and ***P < 0.001; # P < 0.05 NCEXO group vs PA group.

    Article Snippet: After specific cell treatment, the Min6 cell apoptosis rate was performed using an Annexin V and PI apoptosis kit (Proteintech, PF00005) according to the manufacturer’s protocol and then measured by flow cytometry (Becton, Franklin Lake, NJ, USA).

    Techniques: In Vitro, CCK-8 Assay, Flow Cytometry, Expressing, Real-time Polymerase Chain Reaction

    The protective effect of NCEXO primarily stems from its ACE2. ( A ) Protein expression of NLRP3, ASC, Caspase-1, Cleaved Caspase-1, Pro-ILβ, IL1β, IL18, GSDMD and N- GSDMD in mouse pancreatic islets and densitometric quantification. ( B ) Quantitative Real-time PCR was used to measure the level of NLRP3, ASC, Caspase-1, IL1β, IL18, GSDMD of mouse pancreatic islets. ( C ) Quantitative Real-time PCR was used to measure the level of NLRP3, ASC, Caspase-1, IL1β, IL18, GSDMD of Min6 cell. ( D ) The densitometric quantification of Protein expression of NLRP3, ASC, Caspase-1, Cleaved Caspase-1, Pro-ILβ, IL1β, IL18, GSDMD and N- GSDMD in mouse pancreatic islets. ( E ) Quantitative Real-time PCR was used to measure the level of FOXO1, NKX6.1, PDX1, ACE2, NFκB, MAFA of Min6 cell. ( F ) Effect of A779/A1-7 on Min6’cells proliferation measured by EDU. Bar = 100μm ( G-I ) Protein expression of FOXO1, NKX6.1, PDX1, IκB, P-IκB, NFκB, P- NFκB, NLRP3, ASC, Caspase-1, Cleaved Caspase-1, Pro-ILβ, IL1β, IL18, GSDMD and N- GSDMD in Min6 cell. *P < 0.05, **P < 0.01 and ***P < 0.001.

    Journal: International Journal of Nanomedicine

    Article Title: Small Intestinal Endocrine Cell Derived Exosomal ACE2 Protects Islet β-Cell Function by Inhibiting the Activation of NLRP3 Inflammasome and Reducing β-Cell Pyroptosis

    doi: 10.2147/IJN.S450337

    Figure Lengend Snippet: The protective effect of NCEXO primarily stems from its ACE2. ( A ) Protein expression of NLRP3, ASC, Caspase-1, Cleaved Caspase-1, Pro-ILβ, IL1β, IL18, GSDMD and N- GSDMD in mouse pancreatic islets and densitometric quantification. ( B ) Quantitative Real-time PCR was used to measure the level of NLRP3, ASC, Caspase-1, IL1β, IL18, GSDMD of mouse pancreatic islets. ( C ) Quantitative Real-time PCR was used to measure the level of NLRP3, ASC, Caspase-1, IL1β, IL18, GSDMD of Min6 cell. ( D ) The densitometric quantification of Protein expression of NLRP3, ASC, Caspase-1, Cleaved Caspase-1, Pro-ILβ, IL1β, IL18, GSDMD and N- GSDMD in mouse pancreatic islets. ( E ) Quantitative Real-time PCR was used to measure the level of FOXO1, NKX6.1, PDX1, ACE2, NFκB, MAFA of Min6 cell. ( F ) Effect of A779/A1-7 on Min6’cells proliferation measured by EDU. Bar = 100μm ( G-I ) Protein expression of FOXO1, NKX6.1, PDX1, IκB, P-IκB, NFκB, P- NFκB, NLRP3, ASC, Caspase-1, Cleaved Caspase-1, Pro-ILβ, IL1β, IL18, GSDMD and N- GSDMD in Min6 cell. *P < 0.05, **P < 0.01 and ***P < 0.001.

    Article Snippet: After specific cell treatment, the Min6 cell apoptosis rate was performed using an Annexin V and PI apoptosis kit (Proteintech, PF00005) according to the manufacturer’s protocol and then measured by flow cytometry (Becton, Franklin Lake, NJ, USA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction